PrimePCR™ Probe Assay: Pard3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042495
Assay Design:   exonic
Chromosome Location:   8:127389386-127389481question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000162309 ENSMUST00000160272 ENSMUST00000162907 ENSMUST00000162536 ENSMUST00000026921 ENSMUST00000162665 ENSMUST00000160766 ENSMUST00000162602 ENSMUST00000162531 ENSMUST00000160581 ENSMUST00000161355 ENSMUST00000159537 ENSMUST00000161277 ENSMUST00000160717 ENSMUST00000162456 ENSMUST00000162176 ENSMUST00000162727 ENSMUST00000079777 ENSMUST00000108752

Gene Information

Description Not Available

Gene Symbol:   Pard3
Gene Name:   Par-3 (partitioning defective 3) homolog (C. elegans)
Aliases:   AA960621, AI256638, ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000025812
Entrez:   93742
UniGene:   Mm.299254
Chromosome Mapping:   8 E2|8 67.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.760000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download