This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Vegfa, Mouse
PrimePCR™ Template for Probe Assay: Vegfa, Mouse
This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects including mediating increased vascular permeability inducing angiogenesis vasculogenesis endothelial cell growth promoting cell migration and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants give rise to additional isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.