PrimePCR™ Probe Assay: Vegfa, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042478
Assay Design:   exonic
Chromosome Location:   17:46024444-46025360question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000024747 ENSMUST00000071648 ENSMUST00000142351 ENSMUST00000113520 ENSMUST00000113519 ENSMUST00000167860

Gene Information

This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects including mediating increased vascular permeability inducing angiogenesis vasculogenesis endothelial cell growth promoting cell migration and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants give rise to additional isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Vegfa
Gene Name:   Vascular endothelial growth factor A
Aliases:   Vegf, Vegf120, Vegf164, Vegf188, Vpf
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000023951
Entrez:   22339
UniGene:   Mm.282184
Chromosome Mapping:   17 C|17 24.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.710000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download