PrimePCR™ Probe Assay: Lcmt2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042233
Assay Design:   exonic
Chromosome Location:   2:121139056-121139176question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000099486 ENSMUST00000110674

Gene Information

Description Not Available

Gene Symbol:   Lcmt2
Gene Name:   Leucine carboxyl methyltransferase 2
Aliases:   D330024M17, Tyw4
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000074890
Entrez:   329504
UniGene:   Mm.219449
Chromosome Mapping:   2 E5|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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