PrimePCR™ Probe Assay: Crem, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042209
Assay Design:   exonic
Chromosome Location:   18:3273442-3273571question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000151311 ENSMUST00000150235 ENSMUST00000122958 ENSMUST00000154135 ENSMUST00000140332 ENSMUST00000154470 ENSMUST00000129435 ENSMUST00000151084 ENSMUST00000131899 ENSMUST00000130599 ENSMUST00000124747 ENSMUST00000049942 ENSMUST00000127601 ENSMUST00000147138 ENSMUST00000156234 ENSMUST00000152900 ENSMUST00000130455 ENSMUST00000137568 ENSMUST00000146265 ENSMUST00000152108 ENSMUST00000136961 ENSMUST00000144496 ENSMUST00000123672 ENSMUST00000139537 ENSMUST00000154705 ENSMUST00000126578 ENSMUS

Gene Information

This gene encodes a basic-leucine zipper domain-containing protein that localizes to gene promoters where it binds to the cyclic AMP response element (CRE). Different protein isoforms encoded by this gene may function as either activators or repressors of transcription. Activity of this gene is important in multiple developmental processes including spermatogenesis. Mutation of this gene causes male infertility. Alternative splicing and promoter usage result in multiple transcript variants for this gene. [provided by RefSeq Oct 2012]

Gene Symbol:   Crem
Gene Name:   CAMP responsive element modulator
Aliases:   ICER, ICERI
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000063889
Entrez:   12916
UniGene:   Mm.5244
Chromosome Mapping:   18|18 A

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.780000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download