PrimePCR™ Probe Assay: Sgms2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042158
Assay Design:   exonic
Chromosome Location:   3:131342278-131342374question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000090246 ENSMUST00000126569

Gene Information

Sphingomyelin a major component of cell and Golgi membranes is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. [provided by RefSeq Oct 2008]

Gene Symbol:   Sgms2
Gene Name:   Sphingomyelin synthase 2
Aliases:   4933405A16Rik, 5133401H06Rik, AI854299
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000050931
Entrez:   74442
UniGene:   Mm.273360
Chromosome Mapping:   3 G3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.750000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download