PrimePCR™ Probe Assay: Actb, Mouse

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0039589
Assay Design:   Exonic
Chromosome Location:   5:142904472-142904610question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000071555 ENSMUST00000106215 ENSMUST00000075161 ENSMUST00000121731 ENSMUST00000141904 ENSMUST00000078717 ENSMUST00000100497 ENSMUST00000167721 ENSMUST00000171419

Gene Information

Description Not Available

Gene Symbol:   Actb
Gene Name:   Actin, beta
Aliases:   Actx, E430023M04Rik, beta-actin
RefSeq:   NC_000071.6 NT_039316.8
Ensembl:   ENSMUSG00000029580
Entrez:   11461
UniGene:   Mm.391967
Chromosome Mapping   5 G2|5 80.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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