PrimePCR™ Probe Assay: Ang, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0039065
Assay Design:   Exonic
Chromosome Location:   14:51101411-51101602question
Amplicon Length:   162
Splice Variants Targeted:   ENSMUST00000171688 ENSMUST00000069011

Gene Information

This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia mediates stress-induced translational repression by cleaving cellular tRNAs stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease RNase A family 4 gene. Two alternatively spliced variants with different 5' exons but the same coding exon have been identified. Multiple pseudogenes have been found for this gene. [provided by RefSeq Jun 2009]

Gene Symbol:   Ang
Gene Name:   Angiogenin, ribonuclease, RNase A family, 5
Aliases:   AI385586, Ang1, Rnase5, Rnase5a
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000072115
Entrez:   11727
UniGene:   Not Available
Chromosome Mapping:   14 B-C1|14 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 39.570000
Efficiency 84

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download