PrimePCR™ Probe Assay: Dnmt3a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Dnmt3a, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Dnmt3a, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0038538
Assay Design:   Exonic
Chromosome Location:   12:3896031-3896120question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000020991 ENSMUST00000172509 ENSMUST00000174817 ENSMUST00000174102 ENSMUST00000172689 ENSMUST00000174774 ENSMUST00000172749 ENSMUST00000111186

Gene Information

This is one of two related genes encoding de novo DNA methyltransferases which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq Nov 2012]

Gene Symbol:   Dnmt3a
Gene Name:   DNA methyltransferase 3A
Aliases:   AU015806, W91664
RefSeq:   NC_000078.6 NT_039548.8
Ensembl:   ENSMUSG00000020661
Entrez:   13435
Chromosome Mapping:   12|12 A2-A3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.520000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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