PrimePCR™ Probe Assay: Shbg, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0027960
Assay Design:   Exonic
Chromosome Location:   11:69617788-69617887question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000005334

Gene Information

Description Not Available

Gene Symbol:   Shbg
Gene Name:   Sex hormone binding globulin
Aliases:   ABP
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000005202
Entrez:   20415
UniGene:   Mm.1431
Chromosome Mapping:   11 B3|11 35.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.010000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download