PrimePCR™ Probe Assay: 2700073G19Rik, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0027612
Assay Design:   Exonic
Chromosome Location:   12:87442963-87443111question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000091090

Gene Information

Description Not Available

Gene Symbol:   2700073G19Rik
Gene Name:   RIKEN cDNA 2700073G19 gene
Aliases:   Not Available
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000021041
Entrez:   654309
UniGene:   Not Available
Chromosome Mapping:   12 D2|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.995300
y-intercept 34.120000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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