PrimePCR™ SYBR® Green Assay: Vmn1r35, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062966

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061817
Assay Design:   exonic
Chromosome Location:   6:66678807-66678953question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000175988 ENSMUST00000079678 ENSMUST00000071414 ENSMUST00000077482 ENSMUST00000176121

Gene Information

Description Not Available

Gene Symbol:   Vmn1r35
Gene Name:   Vomeronasal 1 receptor 35
Aliases:   V1rc12
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000060699
Entrez:   171185
UniGene:   Mm.377166
Chromosome Mapping:   6 C1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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