PrimePCR™ SYBR® Green Assay: Rhox2h, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062955

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061806
Assay Design:   exonic
Chromosome Location:   X:37672723-37672834question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000113577 ENSMUST00000037708 ENSMUST00000113796 ENSMUST00000166018 ENSMUST00000110043 ENSMUST00000057089 ENSMUST00000093402 ENSMUST00000067549 ENSMUST00000166169 ENSMUST00000074949 ENSMUST00000055097 ENSMUST00000115007 ENSMUST00000115006 ENSMUST00000078111 ENSMUST00000128119 ENSMUST00000159848 ENSMUST00000078470 ENSMUST00000110844 ENSMUST00000113136 ENSMUST00000173804 ENSMUST00000176276 ENSMUST00000078961 ENSMUST00000026296 ENSMUST00000004684 ENSMUST00000045142 ENSMUST00000063340 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rhox2h
Gene Name:   Reproductive homeobox 2H
Aliases:   OTTMUSG00000017156, Rhox2.8, Rhox2g
RefSeq:   NC_000086.7 NT_187037.1
Ensembl:   ENSMUSG00000079627
Entrez:   622301
UniGene:   Mm.350896
Chromosome Mapping:   X A3.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 35.590000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download