PrimePCR™ SYBR® Green Assay: Vmn1r157, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062911

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061762
Assay Design:   exonic
Chromosome Location:   7:22761896-22762042question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000165330 ENSMUST00000079099 ENSMUST00000098747 ENSMUST00000164526 ENSMUST00000168984 ENSMUST00000169774 ENSMUST00000168580 ENSMUST00000172989 ENSMUST00000105209 ENSMUST00000177815 ENSMUST00000164245 ENSMUST00000098744 ENSMUST00000163461 ENSMUST00000169689 ENSMUST00000173886 ENSMUST00000174364 ENSMUST00000179079 ENSMUST00000179511 ENSMUST00000174538 ENSMUST00000173723 ENSMUST00000168794 ENSMUST00000098737 ENSMUST00000169374 ENSMUST00000164288 ENSMUST00000166937 ENSMUST00000172322 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn1r157
Gene Name:   Vomeronasal 1 receptor 157
Aliases:   100043559, EG667551, Gm4517, Gm8699, Vmn1r109
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000095619
Entrez:   667551
UniGene:   Mm.484969
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.740000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download