PrimePCR™ SYBR® Green Assay: Lamtor3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062861

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Lamtor3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Lamtor3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061712
Assay Design:   exonic
Chromosome Location:   3:137928286-137928431question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000179862 ENSMUST00000179801 ENSMUST00000114900 ENSMUST00000114684 ENSMUST00000000201 ENSMUST00000127442 ENSMUST00000144382 ENSMUST00000122899 ENSMUST00000113814 ENSMUST00000120613 ENSMUST00000107596 ENSMUST00000113185 ENSMUST00000064659 ENSMUST00000127348 ENSMUST00000107672 ENSMUST00000107674 ENSMUST00000032968 ENSMUST00000007296 ENSMUST00000168345 ENSMUST00000098312 ENSMUST00000103108 ENSMUST00000097902 ENSMUST00000127984 ENSMUST00000150042 ENSMUST00000084724 ENSMUST00000108127 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Lamtor3
Gene Name:   Late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
Aliases:   AW556229, Map2k1ip1, Mapbp, Mapksp1, Mp1
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000091512
Entrez:   56692
Chromosome Mapping:   3 G3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.950000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download