PrimePCR™ SYBR® Green Assay: CR974575.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062845

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061696
Assay Design:   exonic
Chromosome Location:   12:34290508-34290631question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000161938 ENSMUST00000076842 ENSMUST00000050556 ENSMUST00000170335 ENSMUST00000152407 ENSMUST00000054289 ENSMUST00000146867 ENSMUST00000170715 ENSMUST00000032811 ENSMUST00000094617 ENSMUST00000071458 ENSMUST00000095471 ENSMUST00000165165 ENSMUST00000166546 ENSMUST00000088373 ENSMUST00000113169 ENSMUST00000179802 ENSMUST00000090257 ENSMUST00000112642 ENSMUST00000000449 ENSMUST00000169678 ENSMUST00000085368 ENSMUST00000177599 ENSMUST00000118755 ENSMUST00000094026 ENSMUST00000042647

Gene Information

Description Not Available

Gene Symbol:   CR974575.1
Gene Name:   Not available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095406
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 35.640000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download