PrimePCR™ SYBR® Green Assay: Gdf1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062816

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gdf1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gdf1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061667
Assay Design:   exonic
Chromosome Location:   8:70329996-70330135question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000115799 ENSMUST00000115797 ENSMUST00000040990 ENSMUST00000075510 ENSMUST00000178147 ENSMUST00000024647 ENSMUST00000039476 ENSMUST00000129113 ENSMUST00000100235 ENSMUST00000061450 ENSMUST00000046516 ENSMUST00000165819 ENSMUST00000140239 ENSMUST00000020258

Gene Information

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a monocistronic mRNA early in development and from a bicistronic mRNA in later stages that also encodes the LAG1 homolog ceramide synthase 1 gene. [provided by RefSeq Jul 2009]

Gene Symbol:   Gdf1
Gene Name:   Growth differentiation factor 1
Aliases:   AI385651, Gdf-1
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000055694
Entrez:   14559
Chromosome Mapping:   8 B3.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download