PrimePCR™ SYBR® Green Assay: Vmn2r75, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062773

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn2r75, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn2r75, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061624
Assay Design:   exonic
Chromosome Location:   7:86148227-86148343question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000165834 ENSMUST00000020547 ENSMUST00000170596 ENSMUST00000167976 ENSMUST00000086297 ENSMUST00000163019 ENSMUST00000159674 ENSMUST00000022438 ENSMUST00000159611 ENSMUST00000159734 ENSMUST00000164918 ENSMUST00000094491 ENSMUST00000169805 ENSMUST00000096791 ENSMUST00000071277 ENSMUST00000179115 ENSMUST00000178970 ENSMUST00000099422 ENSMUST00000166327 ENSMUST00000079163 ENSMUST00000095922 ENSMUST00000053253 ENSMUST00000170341 ENSMUST00000167133 ENSMUST00000165180 ENSMUST00000171841 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn2r75
Gene Name:   Vomeronasal 2, receptor 75
Aliases:   EG546981
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000090436
Entrez:   546981
Chromosome Mapping:   7 D3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download