PrimePCR™ SYBR® Green Assay: AC204722.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062750

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC204722.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC204722.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061601
Assay Design:   exonic
Chromosome Location:   Y:13376800-13376924question
Amplicon Length:   95
Splice Variants Targeted:   ENSMUST00000087186 ENSMUST00000178599 ENSMUST00000177639 ENSMUST00000089534 ENSMUST00000045827 ENSMUST00000169190 ENSMUST00000047281 ENSMUST00000105786 ENSMUST00000078886 ENSMUST00000100882 ENSMUST00000033490 ENSMUST00000072119 ENSMUST00000091295 ENSMUST00000045557 ENSMUST00000029766 ENSMUST00000181081

Gene Information

Description Not Available

Gene Symbol:   AC204722.1
Gene Name:   Y-linked testis-specific protein 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000093903
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download