PrimePCR™ SYBR® Green Assay: Defa-rs1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062734

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061585
Assay Design:   exonic
Chromosome Location:   8:21325939-21326114question
Amplicon Length:   146
Splice Variants Targeted:   ENSMUST00000084843 ENSMUST00000094129 ENSMUST00000131446 ENSMUST00000068700 ENSMUST00000161017 ENSMUST00000160789 ENSMUST00000059817 ENSMUST00000117095 ENSMUST00000117611 ENSMUST00000098890 ENSMUST00000110752 ENSMUST00000110749 ENSMUST00000071886 ENSMUST00000102801 ENSMUST00000057207 ENSMUST00000163524 ENSMUST00000107590 ENSMUST00000156951 ENSMUST00000055537 ENSMUST00000053218 ENSMUST00000054356 ENSMUST00000028155 ENSMUST00000113869 ENSMUST00000113867 ENSMUST00000113870 ENSMUST00000094203 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Defa-rs1
Gene Name:   Defensin, alpha, related sequence 1
Aliases:   2010300L12Rik, 2010319H24Rik, CRS1C, CRS1C-2, CRS1C-5, Defcr-rs1
RefSeq:   NC_000074.6 NT_039457.8
Ensembl:   ENSMUSG00000074437
Entrez:   13218
UniGene:   Mm.304264
Chromosome Mapping:   8 A2|8 9.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download