PrimePCR™ SYBR® Green Assay: Mfap1b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062684

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061535
Assay Design:   exonic
Chromosome Location:   2:121469885-121470024question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000066509 ENSMUST00000114608 ENSMUST00000114627 ENSMUST00000094787 ENSMUST00000041197 ENSMUST00000180359 ENSMUST00000179669 ENSMUST00000142443 ENSMUST00000165379 ENSMUST00000141911 ENSMUST00000170618 ENSMUST00000038753 ENSMUST00000107586 ENSMUST00000056732 ENSMUST00000089926 ENSMUST00000084241 ENSMUST00000105912 ENSMUST00000047075 ENSMUST00000047157 ENSMUST00000067252 ENSMUST00000031694

Gene Information

Description Not Available

Gene Symbol:   Mfap1b
Gene Name:   Microfibrillar-associated protein 1B
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000048222
Entrez:   100034361
UniGene:   Mm.270393
Chromosome Mapping:   2 F1|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.300000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download