PrimePCR™ SYBR® Green Assay: Ifna1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062630

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061481
Assay Design:   exonic
Chromosome Location:   4:88850133-88850231question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000111837 ENSMUST00000032362 ENSMUST00000023532 ENSMUST00000102809 ENSMUST00000102808 ENSMUST00000102807 ENSMUST00000102806 ENSMUST00000105149 ENSMUST00000072734 ENSMUST00000105148 ENSMUST00000105147 ENSMUST00000105146 ENSMUST00000105143 ENSMUST00000170428 ENSMUST00000071378 ENSMUST00000102804 ENSMUST00000094973 ENSMUST00000094972 ENSMUST00000021479

Gene Information

Description Not Available

Gene Symbol:   Ifna1
Gene Name:   Interferon alpha 1
Aliases:   Ifa1
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000095498
Entrez:   15962
UniGene:   Mm.57127
Chromosome Mapping:   4 C4|4 42.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download