PrimePCR™ SYBR® Green Assay: AC190166.2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062582

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061433
Assay Design:   exonic
Chromosome Location:   Y:13133183-13133322question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000179125 ENSMUST00000177579 ENSMUST00000178559 ENSMUST00000177612 ENSMUST00000177878 ENSMUST00000178669 ENSMUST00000179612 ENSMUST00000179284 ENSMUST00000178050 ENSMUST00000178519 ENSMUST00000178428 ENSMUST00000178689 ENSMUST00000179522 ENSMUST00000179900 ENSMUST00000179219 ENSMUST00000179302

Gene Information

Description Not Available

Gene Symbol:   AC190166.2
Gene Name:   Serine-rich secreted Y-linked protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095769
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.050000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download