PrimePCR™ SYBR® Green Assay: Ifna2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062575

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061426
Assay Design:   exonic
Chromosome Location:   4:88683528-88683669question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000047786 ENSMUST00000160844 ENSMUST00000160800 ENSMUST00000159778 ENSMUST00000174337 ENSMUST00000172466 ENSMUST00000054059 ENSMUST00000161402 ENSMUST00000174331 ENSMUST00000072671 ENSMUST00000176174 ENSMUST00000084731 ENSMUST00000115048 ENSMUST00000115046 ENSMUST00000102895 ENSMUST00000087400 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000112931 ENSMUST00000112930 ENSMUST00000130732 ENSMUST00000113876 ENSMUST00000113883 ENSMUST00000113882 ENSMUST00000113878 ENSMUST00000093942 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ifna2
Gene Name:   Interferon alpha 2
Aliases:   Ifa2
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000078354
Entrez:   15965
UniGene:   Mm.14091
Chromosome Mapping:   4 C4|4 42.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download