PrimePCR™ SYBR® Green Assay: Txnrd3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062142

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Txnrd3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Txnrd3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0060993
Assay Design:   exonic
Chromosome Location:   6:89651593-89654139question
Amplicon Length:   72
Splice Variants Targeted:   ENSMUST00000000828 ENSMUST00000101171

Gene Information

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein catalyzes the reduction of thioredoxin but unlike other mammalian thioredoxin reductases (TRs) it contains an additional glutaredoxin domain and shows highest expression in testes. Like other TRs it contains a C-terminal penultimate selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG leading to additional isoforms. [provided by RefSeq May 2010]

Gene Symbol:   Txnrd3
Gene Name:   Thioredoxin reductase 3
Aliases:   AI196535, TR2, Tgr
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000000811
Entrez:   232223
Chromosome Mapping:   6 D1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download