PrimePCR™ SYBR® Green Assay: Myl6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060677

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052253
Assay Design:   exonic
Chromosome Location:   10:128490922-128492062question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000132517 ENSMUST00000171637 ENSMUST00000043866 ENSMUST00000053175 ENSMUST00000100721 ENSMUST00000170242 ENSMUST00000168839 ENSMUST00000071935 ENSMUST00000001583 ENSMUST00000091851 ENSMUST00000159162 ENSMUST00000026494 ENSMUST00000117100 ENSMUST00000022851 ENSMUST00000022036 ENSMUST00000055539 ENSMUST00000105244 ENSMUST00000105242 ENSMUST00000105245 ENSMUST00000164181 ENSMUST00000056915 ENSMUST00000042850 ENSMUST00000002452 ENSMUST00000106804 ENSMUST00000106803 ENSMUST00000068693 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Myl6
Gene Name:   Myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
Aliases:   ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3SM, MLC3nm, Myln
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000090841
Entrez:   17904
UniGene:   Mm.337074
Chromosome Mapping:   10 D3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.650000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download