PrimePCR™ SYBR® Green Assay: Zfp882, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0060670

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Zfp882, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Zfp882, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052246
Assay Design:   exonic
Chromosome Location:   8:71908721-71913234question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000072465 ENSMUST00000170101 ENSMUST00000034259 ENSMUST00000110002 ENSMUST00000125802 ENSMUST00000126607 ENSMUST00000131237 ENSMUST00000136516 ENSMUST00000132848 ENSMUST00000109997 ENSMUST00000085664 ENSMUST00000105316 ENSMUST00000076281 ENSMUST00000105314 ENSMUST00000041264 ENSMUST00000107989 ENSMUST00000115861 ENSMUST00000047322 ENSMUST00000167516 ENSMUST00000148442 ENSMUST00000146074 ENSMUST00000074322 ENSMUST00000177931 ENSMUST00000115333 ENSMUST00000115334 ENSMUST00000040628 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Zfp882
Gene Name:   Zinc finger protein 882
Aliases:   ENSMUSG00000052439, Gm15468, OTTMUSG00000022410
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000089857
Entrez:   382019
Chromosome Mapping:   8 B3.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download