PrimePCR™ SYBR® Green Assay: Cpne1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0060630

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052205
Assay Design:   exonic
Chromosome Location:   2:156078408-156078649question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000096069 ENSMUST00000111062 ENSMUST00000022660 ENSMUST00000159840 ENSMUST00000160884 ENSMUST00000030768 ENSMUST00000071093 ENSMUST00000106283 ENSMUST00000057167 ENSMUST00000109608 ENSMUST00000109607 ENSMUST00000079312 ENSMUST00000136296 ENSMUST00000153634 ENSMUST00000142960 ENSMUST00000133921

Gene Information

This gene encodes a protein that contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. Its activity is also upregulated in mouse embryos. This gene and the gene for RNA binding motif protein 12 overlap at map location 2 H2. Two alternatively spliced variants that encode the same isoform have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Cpne1
Gene Name:   Copine I
Aliases:   1810028N16Rik, mKIAA4108
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000074643
Entrez:   266692
UniGene:   Not Available
Chromosome Mapping:   2 H2|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.660000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download