PrimePCR™ SYBR® Green Assay: Klra10, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0060625

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052200
Assay Design:   exonic
Chromosome Location:   6:130281827-130281927question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000110081 ENSMUST00000110078 ENSMUST00000122219 ENSMUST00000014687 ENSMUST00000118060 ENSMUST00000014683 ENSMUST00000074056 ENSMUST00000112032 ENSMUST00000071554 ENSMUST00000049304 ENSMUST00000088011 ENSMUST00000032286 ENSMUST00000112013 ENSMUST00000112020 ENSMUST00000111998 ENSMUST00000088017 ENSMUST00000032288 ENSMUST00000032306 ENSMUST00000088867 ENSMUST00000061201 ENSMUST00000043983 ENSMUST00000043325

Gene Information

Description Not Available

Gene Symbol:   Klra10
Gene Name:   Killer cell lectin-like receptor subfamily A, member 10
Aliases:   Ly49J, Ly49i2
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000072718
Entrez:   16628
UniGene:   Mm.445916
Chromosome Mapping:   6 F3|6 62.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download