PrimePCR™ SYBR® Green Assay: Fcho1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060610

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Fcho1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fcho1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052185
Assay Design:   exonic
Chromosome Location:   8:71718295-71721257question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000146100 ENSMUST00000153800 ENSMUST00000125092 ENSMUST00000093444 ENSMUST00000005616 ENSMUST00000144258 ENSMUST00000097949 ENSMUST00000062353 ENSMUST00000060655 ENSMUST00000057974 ENSMUST00000053484 ENSMUST00000061890 ENSMUST00000072141 ENSMUST00000023119 ENSMUST00000057093 ENSMUST00000021554 ENSMUST00000096633 ENSMUST00000016771

Gene Information

Description Not Available

Gene Symbol:   Fcho1
Gene Name:   FCH domain only 1
Aliases:   3322402E17Rik, N28152
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000070000
Entrez:   74015
Chromosome Mapping:   8 B3.3|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download