PrimePCR™ SYBR® Green Assay: Cdc42ep5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0060584

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0052159
Assay Design:   exonic
Chromosome Location:   7:4151676-4151781question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000076831 ENSMUST00000143825 ENSMUST00000076896 ENSMUST00000025862 ENSMUST00000176030 ENSMUST00000176769 ENSMUST00000021296 ENSMUST00000029679 ENSMUST00000107422

Gene Information

Description Not Available

Gene Symbol:   Cdc42ep5
Gene Name:   CDC42 effector protein (Rho GTPase binding) 5
Aliases:   1700027J19Rik, 2010007O02Rik, Borg3, C85526, CEP5
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000063838
Entrez:   58804
UniGene:   Mm.34268
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download