PrimePCR™ SYBR® Green Assay: Tmsb15l, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0060312

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0051886
Assay Design:   exonic
Chromosome Location:   X:136955228-136955354question
Amplicon Length:   97
Splice Variants Targeted:   ENSMUST00000127404

Gene Information

This record represents readthrough transcripts derived from two adjacent genes represented by GeneIDs 100034363 and 666244. Each gene has three exons. The readthrough transcripts include two exons from each gene such that the second exon is represented twice although not identically in the readthrough transcripts. [provided by RefSeq Jul 2008]

Gene Symbol:   Tmsb15l
Gene Name:   Thymosin beta 15b like
Aliases:   4930488E11Rik, Tb15r, Tmsb15b1-Tmsb15b2, Tmsb15r
RefSeq:   NC_000086.7 NT_039716.8
Ensembl:   ENSMUSG00000072955
Entrez:   399591
UniGene:   Mm.381904
Chromosome Mapping:   X F1|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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