This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0060312
PrimePCR™ PreAmp for SYBR® Green Assay: Tmsb15l, Mouse
PrimePCR™ Template for SYBR® Green Assay: Tmsb15l, Mouse
This record represents readthrough transcripts derived from two adjacent genes represented by GeneIDs 100034363 and 666244. Each gene has three exons. The readthrough transcripts include two exons from each gene such that the second exon is represented twice although not identically in the readthrough transcripts. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.