This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059538
PrimePCR™ PreAmp for SYBR® Green Assay: Inadl, Mouse
PrimePCR™ Template for SYBR® Green Assay: Inadl, Mouse
This gene encodes a multivalent PDZ domain protein which is expressed exclusively in brain and kidney. This protein selectively interacts with inward rectifier K+ (Kir) family members N-methyl-D-aspartate receptor subunits neurexins and neuroligins as well as cell surface molecules enriched in synaptic membranes. Thus this protein may serve as a scaffold that brings structurally diverse but functionally connected proteins into close proximity at the synapse. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.