PrimePCR™ SYBR® Green Assay: Inadl, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059538

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Inadl, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Inadl, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0051112
Assay Design:   exonic
Chromosome Location:   4:98674242-98674334question
Amplicon Length:   63
Splice Variants Targeted:   ENSMUST00000041284 ENSMUST00000107034 ENSMUST00000107029 ENSMUST00000102792 ENSMUST00000030290

Gene Information

This gene encodes a multivalent PDZ domain protein which is expressed exclusively in brain and kidney. This protein selectively interacts with inward rectifier K+ (Kir) family members N-methyl-D-aspartate receptor subunits neurexins and neuroligins as well as cell surface molecules enriched in synaptic membranes. Thus this protein may serve as a scaffold that brings structurally diverse but functionally connected proteins into close proximity at the synapse. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   Inadl
Gene Name:   InaD-like (Drosophila)
Aliases:   Cipp, Patj
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000061859
Entrez:   12695
Chromosome Mapping:   4 C6|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 38.760000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download