PrimePCR™ SYBR® Green Assay: C87977, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059306

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050880
Assay Design:   exonic
Chromosome Location:   4:144209400-144209542question
Amplicon Length:   113
Splice Variants Targeted:   ENSMUST00000095509 ENSMUST00000180803 ENSMUST00000180707 ENSMUST00000181843 ENSMUST00000180706 ENSMUST00000181394 ENSMUST00000110145 ENSMUST00000181326 ENSMUST00000181300 ENSMUST00000061277 ENSMUST00000073641 ENSMUST00000085144 ENSMUST00000105770 ENSMUST00000105754 ENSMUST00000105753 ENSMUST00000105757 ENSMUST00000147855 ENSMUST00000146836 ENSMUST00000071580 ENSMUST00000180552 ENSMUST00000180702

Gene Information

Description Not Available

Gene Symbol:   C87977
Gene Name:   Expressed sequence C87977
Aliases:   C85819
RefSeq:   NC_000070.6 NT_039291.1 NT_187033.1
Ensembl:   ENSMUSG00000046262
Entrez:   97187
UniGene:   Mm.422698
Chromosome Mapping:   4 E1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.110000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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