PrimePCR™ SYBR® Green Assay: Scml4, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059303

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050877
Assay Design:   exonic
Chromosome Location:   10:42912202-42924162question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000106162 ENSMUST00000030673 ENSMUST00000030170 ENSMUST00000163852 ENSMUST00000126319 ENSMUST00000077816 ENSMUST00000022629 ENSMUST00000023779 ENSMUST00000063063 ENSMUST00000157071 ENSMUST00000105495 ENSMUST00000105494 ENSMUST00000136841 ENSMUST00000125576 ENSMUST00000030187 ENSMUST00000087978 ENSMUST00000035371

Gene Information

Description Not Available

Gene Symbol:   Scml4
Gene Name:   Sex comb on midleg-like 4 (Drosophila)
Aliases:   9330161D17, mFLJ00197
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000044770
Entrez:   268297
UniGene:   Mm.98731
Chromosome Mapping:   10 B2|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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