PrimePCR™ SYBR® Green Assay: Nrf1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059283

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050857
Assay Design:   exonic
Chromosome Location:   6:30116156-30116266question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000115212 ENSMUST00000115211 ENSMUST00000115206 ENSMUST00000115208 ENSMUST00000115209 ENSMUST00000115200 ENSMUST00000115204 ENSMUST00000115199 ENSMUST00000132866 ENSMUST00000167972 ENSMUST00000069808 ENSMUST00000170535 ENSMUST00000069831 ENSMUST00000004761

Gene Information

Description Not Available

Gene Symbol:   Nrf1
Gene Name:   Nuclear respiratory factor 1
Aliases:   C87038, D6Ertd415e
RefSeq:   NC_000072.6 NT_039340.7
Ensembl:   ENSMUSG00000058440
Entrez:   18181
UniGene:   Mm.259258
Chromosome Mapping:   6 A3.3|6 6.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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