PrimePCR™ SYBR® Green Assay: Suv420h1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059099

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0050673
Assay Design:   exonic
Chromosome Location:   19:3786626-3793184question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000113974 ENSMUST00000113972 ENSMUST00000113973 ENSMUST00000113968 ENSMUST00000113970 ENSMUST00000113967 ENSMUST00000152935 ENSMUST00000176262 ENSMUST00000176407 ENSMUST00000176926 ENSMUST00000176512 ENSMUST00000177355 ENSMUST00000113977 ENSMUST00000052699 ENSMUST00000005518

Gene Information

Description Not Available

Gene Symbol:   Suv420h1
Gene Name:   Suppressor of variegation 4-20 homolog 1 (Drosophila)
Aliases:   AA117471, C630029K18Rik, KMT5B, Suv4-20h1
RefSeq:   NC_000085.6 NT_082868.6
Ensembl:   ENSMUSG00000045098
Entrez:   225888
UniGene:   Mm.278578
Chromosome Mapping:   19 A|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.220000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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