PrimePCR™ SYBR® Green Assay: Abi1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057999

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049573
Assay Design:   exonic
Chromosome Location:   2:22957090-22960818question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000140164 ENSMUST00000126112 ENSMUST00000149719 ENSMUST00000123948 ENSMUST00000091394 ENSMUST00000153931 ENSMUST00000139038 ENSMUST00000114544 ENSMUST00000078977 ENSMUST00000093171 ENSMUST00000178908

Gene Information

Description Not Available

Gene Symbol:   Abi1
Gene Name:   Abl-interactor 1
Aliases:   E3B1, NAP1, Ssh3bp1
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000058835
Entrez:   11308
UniGene:   Mm.205647
Chromosome Mapping:   2 A3|2 15.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.370000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download