PrimePCR™ SYBR® Green Assay: Rgs8, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057973

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rgs8, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rgs8, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049547
Assay Design:   exonic
Chromosome Location:   1:153690907-153691021question
Amplicon Length:   85
Splice Variants Targeted:   ENSMUST00000060693 ENSMUST00000031590 ENSMUST00000106447 ENSMUST00000100069 ENSMUST00000066432 ENSMUST00000041776 ENSMUST00000124500 ENSMUST00000111815 ENSMUST00000152114 ENSMUST00000111812 ENSMUST00000147700 ENSMUST00000147482 ENSMUST00000111814 ENSMUST00000111810 ENSMUST00000027748 ENSMUST00000061274 ENSMUST00000089622 ENSMUST00000067485 ENSMUST00000090071 ENSMUST00000011934 ENSMUST00000099981 ENSMUST00000111882 ENSMUST00000111846

Gene Information

Description Not Available

Gene Symbol:   Rgs8
Gene Name:   Regulator of G-protein signaling 8
Aliases:   6530413N01Rik
RefSeq:   NC_000067.6 NT_078297.7
Ensembl:   ENSMUSG00000042671
Entrez:   67792
Chromosome Mapping:   1 G3|1 78.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download