PrimePCR™ SYBR® Green Assay: Gemin8, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057960

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049534
Assay Design:   exonic
Chromosome Location:   X:166180685-166180796question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000019708 ENSMUST00000105377 ENSMUST00000105376 ENSMUST00000111464 ENSMUST00000090682 ENSMUST00000035960 ENSMUST00000110071 ENSMUST00000105991 ENSMUST00000064099 ENSMUST00000076939 ENSMUST00000160180 ENSMUST00000069922 ENSMUST00000027898 ENSMUST00000097442 ENSMUST00000025036 ENSMUST00000086835 ENSMUST00000047721 ENSMUST00000036407 ENSMUST00000058960 ENSMUST00000050471 ENSMUST00000035208 ENSMUST00000038671 ENSMUST00000032132 ENSMUST00000020441 ENSMUST00000036579 ENSMUST00000109456 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gemin8
Gene Name:   Gem (nuclear organelle) associated protein 8
Aliases:   B130034M20Rik, B930082C09Rik, BC023488, gemin-8
RefSeq:   NC_000086.7 NT_039718.8
Ensembl:   ENSMUSG00000040621
Entrez:   237221
UniGene:   Mm.209490
Chromosome Mapping:   X F5|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download