PrimePCR™ SYBR® Green Assay: Klhdc4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0057958

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049532
Assay Design:   exonic
Chromosome Location:   8:121821357-121829390question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000003469 ENSMUST00000003370 ENSMUST00000109799 ENSMUST00000004156 ENSMUST00000045884 ENSMUST00000174717 ENSMUST00000029510 ENSMUST00000103119 ENSMUST00000105113 ENSMUST00000108901 ENSMUST00000087563 ENSMUST00000051772 ENSMUST00000074834 ENSMUST00000023226 ENSMUST00000072692 ENSMUST00000169438 ENSMUST00000169714 ENSMUST00000169108 ENSMUST00000076442 ENSMUST00000171562 ENSMUST00000165210 ENSMUST00000089610 ENSMUST00000071869 ENSMUST00000073418 ENSMUST00000054449 ENSMUST00000080857 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Klhdc4
Gene Name:   Kelch domain containing 4
Aliases:   AA408426, AV352552, BC012312, G430025P05Rik
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000040263
Entrez:   234825
UniGene:   Mm.209329
Chromosome Mapping:   8 E1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.210000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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