PrimePCR™ SYBR® Green Assay: Tle2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057931

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0049505
Assay Design:   exonic
Chromosome Location:   10:81575595-81575688question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000120204 ENSMUST00000106017 ENSMUST00000121049 ENSMUST00000106015 ENSMUST00000106018 ENSMUST00000097868 ENSMUST00000030571 ENSMUST00000131794 ENSMUST00000136341 ENSMUST00000135211 ENSMUST00000146916 ENSMUST00000143285 ENSMUST00000146358 ENSMUST00000105616 ENSMUST00000030940 ENSMUST00000176637 ENSMUST00000165335 ENSMUST00000102787 ENSMUST00000028161

Gene Information

Description Not Available

Gene Symbol:   Tle2
Gene Name:   Transducin-like enhancer of split 2, homolog of Drosophila E(spl)
Aliases:   Grg2, mKIAA4188
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000034771
Entrez:   21886
UniGene:   Mm.38608
Chromosome Mapping:   10 C1|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 34.880000
Efficiency 109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download