This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0057798
PrimePCR™ PreAmp for SYBR® Green Assay: Wdr41, Mouse
PrimePCR™ Template for SYBR® Green Assay: Wdr41, Mouse
This gene encodes a protein of unknown function but which contains a WD40 domain consisting of six WD40 repeats. The WD40 domain is one of the most abundant protein domains in eukaryotes and is found in proteins with widely varying cellular functions. However proteins with this domain often provide a rigid scaffold for protein-protein interactions. [provided by RefSeq Aug 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.