PrimePCR™ SYBR® Green Assay: Abcg4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0056578

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048152
Assay Design:   exonic
Chromosome Location:   9:44287245-44288014question
Amplicon Length:   192
Splice Variants Targeted:   ENSMUST00000066285 ENSMUST00000034648 ENSMUST00000161354 ENSMUST00000161408 ENSMUST00000160384 ENSMUST00000101377 ENSMUST00000101382 ENSMUST00000101376 ENSMUST00000069809 ENSMUST00000080673 ENSMUST00000091818 ENSMUST00000088246 ENSMUST00000084954 ENSMUST00000108568 ENSMUST00000079056 ENSMUST00000102564 ENSMUST00000124943 ENSMUST00000150076 ENSMUST00000102563 ENSMUST00000080036

Gene Information

Description Not Available

Gene Symbol:   Abcg4
Gene Name:   ATP-binding cassette, sub-family G (WHITE), member 4
Aliases:   6430517O04Rik
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000032131
Entrez:   192663
UniGene:   Mm.101876
Chromosome Mapping:   9|9 A5.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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