PrimePCR™ SYBR® Green Assay: Tceal6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0056573

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048147
Assay Design:   exonic
Chromosome Location:   X:135209945-135210612question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000078451 ENSMUST00000119499 ENSMUST00000121409 ENSMUST00000020411 ENSMUST00000025425 ENSMUST00000062964 ENSMUST00000023812 ENSMUST00000171565 ENSMUST00000169377 ENSMUST00000160466 ENSMUST00000161966 ENSMUST00000027817 ENSMUST00000145167 ENSMUST00000160946 ENSMUST00000161132 ENSMUST00000161795 ENSMUST00000098979 ENSMUST00000030029 ENSMUST00000035208 ENSMUST00000000924 ENSMUST00000006626 ENSMUST00000002976 ENSMUST00000029603 ENSMUST00000108689 ENSMUST00000165221 ENSMUST00000007301 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tceal6
Gene Name:   Transcription elongation factor A (SII)-like 6
Aliases:   1500026B10Rik
RefSeq:   NC_000086.7 NT_039716.8
Ensembl:   ENSMUSG00000031409
Entrez:   66104
UniGene:   Mm.336239
Chromosome Mapping:   X F1|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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