PrimePCR™ SYBR® Green Assay: Tead2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0056567

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048141
Assay Design:   exonic
Chromosome Location:   7:45217238-45217333question
Amplicon Length:   66
Splice Variants Targeted:   ENSMUST00000035960 ENSMUST00000109104 ENSMUST00000071750 ENSMUST00000180083 ENSMUST00000032811 ENSMUST00000094617 ENSMUST00000033060 ENSMUST00000155313 ENSMUST00000107801 ENSMUST00000134420 ENSMUST00000097216 ENSMUST00000112835 ENSMUST00000143610 ENSMUST00000030469 ENSMUST00000021424 ENSMUST00000028944 ENSMUST00000000466 ENSMUST00000152564 ENSMUST00000057272 ENSMUST00000099467 ENSMUST00000123490 ENSMUST00000020662 ENSMUST00000015628 ENSMUST00000053287

Gene Information

Description Not Available

Gene Symbol:   Tead2
Gene Name:   TEA domain family member 2
Aliases:   ETF, Etdf, TEAD-2, TEF-4, TEF4
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000030796
Entrez:   21677
UniGene:   Mm.3019
Chromosome Mapping:   7 B5|7 23.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.900000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download