PrimePCR™ SYBR® Green Assay: Nkx1-1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0056556

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048130
Assay Design:   exonic
Chromosome Location:   5:33431427-33431526question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000099552 ENSMUST00000101655 ENSMUST00000081318 ENSMUST00000066391 ENSMUST00000153425 ENSMUST00000140846 ENSMUST00000006397 ENSMUST00000039229 ENSMUST00000084806 ENSMUST00000113130 ENSMUST00000061013 ENSMUST00000044387 ENSMUST00000144079 ENSMUST00000074157 ENSMUST00000121875 ENSMUST00000128119 ENSMUST00000105286 ENSMUST00000001845 ENSMUST00000064454 ENSMUST00000111234 ENSMUST00000111236 ENSMUST00000118174 ENSMUST00000117422 ENSMUST00000119547 ENSMUST00000117488 ENSMUST00000105328 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Nkx1-1
Gene Name:   NK1 transcription factor related, locus 1 (Drosophila)
Aliases:   Nkx-1.1, Sax2
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000029112
Entrez:   672284
UniGene:   Not Available
Chromosome Mapping:   5 B1|5 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.480000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download