PrimePCR™ SYBR® Green Assay: Commd8, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0056068

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0047642
Assay Design:   exonic
Chromosome Location:   5:72159669-72159778question
Amplicon Length:   80
Splice Variants Targeted:   ENSMUST00000013693

Gene Information

Description Not Available

Gene Symbol:   Commd8
Gene Name:   COMM domain containing 8
Aliases:   C77770, D5Buc26e
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000029213
Entrez:   27784
UniGene:   Mm.44223
Chromosome Mapping:   5 C3.2|5 40.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.190000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download