This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0055975
PrimePCR™ PreAmp for SYBR® Green Assay: Abhd10, Mouse
PrimePCR™ Template for SYBR® Green Assay: Abhd10, Mouse
This gene encodes a mitochondrially-localized enzyme that acts as a hydrolase. The encoded protein removes glucuronide from mycophenolic acid acyl-glucuronide. There are two pseudogenes for this gene on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2013]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.