This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0055758
PrimePCR™ PreAmp for SYBR® Green Assay: Rchy1, Mouse
PrimePCR™ Template for SYBR® Green Assay: Rchy1, Mouse
This gene encodes a protein containing CHY- CTCHY- and RING-type zinc-fingers. The encoded protein functions as an E3 ubiquitin ligase and mediates the degradation of target proteins such as p53. The activity of this protein is important in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.