PrimePCR™ SYBR® Green Assay: Commd9, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0055236

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Commd9, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Commd9, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046810
Assay Design:   exonic
Chromosome Location:   2:101901108-101901257question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000030170 ENSMUST00000177517 ENSMUST00000025425 ENSMUST00000109553 ENSMUST00000023024 ENSMUST00000079978 ENSMUST00000163653 ENSMUST00000107952 ENSMUST00000107953 ENSMUST00000106763 ENSMUST00000106762 ENSMUST00000057892 ENSMUST00000000291 ENSMUST00000106715 ENSMUST00000106718 ENSMUST00000105907 ENSMUST00000028584

Gene Information

Description Not Available

Gene Symbol:   Commd9
Gene Name:   COMM domain containing 9
Aliases:   1810029F08Rik, AA409293
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027163
Entrez:   76501
Chromosome Mapping:   2 E2|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.810000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download